Supplemental Notes for Chapter 2 Basic Chemistry Atomic number - number of protons Atomic mass - number of protons and neutrons - unit of atomic mass is daltons (d), 1d mass of H atom - 1 gram atomic mass of any element = 6X1023 atoms Molecular weight - weight expressed in atomic mass units or daltons (MW). - weight of a mole of atoms or molecules. - 1 gram molecular mass = 1 mole = 6X1023 atoms - glucose (C6H12O6) has a gram molecular weight of 180 daltons Shells - energy levels that electrons occupy. - divided into sublevels;- sublevels divided into orbitals; - orbitals hold a max of 2 electrons. Radioisotopes - unstable isotopes (neutron # differs) that emit radiation - Common isotopes used in biomedical research and clinical medicine;- 32P, 35S, 3H, 125I. - use of radioisotopes in medicine known as nuclear medicine. Free radicals - atom containing a single electron in its outer orbital. - React rapidly to fill unpaired orbital, thus exist for brief periods. - Common biological examples, superoxide anion O2-, hydroxyl OH, and nitric oxide NO. Covalent bonds - stongest chemical bond between two atoms - extremely important bond for producing protein structure, storing and liberating energy - number of possible bonds for the four most abundant atoms in the body: Hydrogen - one, Oxygen - two, Nitrogen - three, Carbon - four Body Composition : 99.3% H, O, N, C 0.7% essential minerals (Ca, P, K, S, Na, Cl, Mg) 0.01% Trace elements (Fe, I, Cu, Zn, Mn, Co, Cr, Se, Mo, F, Sn, Si, V) Ionic bonds - formed when atoms gain or lose an electron to become - - cations (positive) or anions (negative) - ions in solution are called electrolytes - bonds between opposite charges of a pair of ions are called ionic bonds. - do not involve sharing an electron as in covalent bonds. Hydrogen bonds: weak bonds between positive part of hydrogen and negative regions of polar molecules (areas with high O or N density). -While weak, these bonds are very important in determining molecular shape for most biological molecules. Van der Waals forces - even weaker bonds than hydrogen bonds - nonspecific attractions between the positive nucleus of any atom and the electrons of nearby atoms - important in nonpolar regions - important for intramolecular packing and thus supplement H bonds. Polar Molecules - molecules with an uneven distribution of charge. - some elements, particularly N and O, have a strong attraction for electrons - causes an uneven distribution of charge, with a positive end and a more negative end. - can occur across a single bond, or across a very large molecule. - OTOH, non-polar molecules have a fairly even distribution of charge Water : The chemistry of water is crucial to the functioning of living systems - out of every 100 molecules in body, 99 are water. -Water is a polar molecule. -Water forms hydrogen bonds with itself (H to O), leads to surface tension -Water molecules take part in many chemical reactions in living systems Solutions: Solute = what dissolves, Solvent = liquid in which solute dissolves. -In living systems, solvent is water - aqueous solution -To dissolve, a substance must be electrically attracted to water. -Polar molecules dissolve easily and are termed Hydrophilic -Non-polar molecules do not dissolve easily, and are termed Hydrophobic -Some large molecules are polar on one end, and non-polar on the other and are termed Amphipathic. e.g. phospholipids Acidity : Acidity refers to the concentration of free protons in solution - too much H+ can interfere with hydrogen bonding, van der Walls forces, and thus produce large changes in molecular interactions. - solutions are either acidic (high H+) or alkaline (low H+). - molecules that release H+ (protons) are called acids e.g. HCl = H+ + Cl- CO2 + H2O = H2CO3 = H+ + HCO3- Carbonic acid bicarbonate - buffer is any molecule that helps prevent large changes in H+ -Substances that accept a hydrogen ion are called a base -Acids differ in their propensity to release protons. Acidity is expressed with the pH scale where pH = -log10 [H+] - solution with [H+] = 10-7M has pH = 7 - lower pH is more acidic, higher pH is more basic