ASSIGNMENT # 2            RESPONSE SHEET       NAMES -
Bring to class Sept 20.
NO EXTENSIONS

Problem #1 [RIBONUCLEASE]

1.  How many beta sheets are indicated?
    How many alpha helices?

    What motifs are present?


2.  Which features (in the diagram) contribute to tertiary structure?

    Which amino acids are involved?

3.  Which amino acids are part of the catalytic site?


Problem # 2  [MYOGLOBIN]

1.  What is the historical significance of this molecule?


2.  The function of the molecule is given.  What would be the 
    physiological significance/importance of this molecule when exercising?



3.  What method was employed by these investigators to study this protein?


4.  Would you expect this protein to be fibrous or globular?
     What data supports your selection?

5.  What is the 1st and last amino acid in the alpha helix nearest 
     the C terminus of the molecule -
     the N terminus -
 
Problem #3   [PEPSIN]

1.  How does the cell protect itself from autolysis by pepsin?



2.  Reactive sites of enzymes must bind the "appropriate" substrate.
    Would you expect a reactive site to be near the interior or the 
     exterior of the enzyme?

Problem #4  [ALCOHOL DEHYDROGENASE]

1.  Why is this protein, like hemogloblin, a good example to use to 
     illustrate quarternary structure?


2.  How much similarity exists between the chains?  


    How might this degree of similarity happen?